French revolutionists condemned King Louis XVI to death on 21. January 1793 by means of the guillotine at the Place de la Revolution in Paris (roughly where the Obelisk decorating the Place de la Concorde stands today). After a short but defiant speech he lost his head as the crowd rushed to the scaffold to dip hankerchiefs into his blood as momentos. An ornate gourd decorated with French Revolution themes was recently uncovered which had contained a blood soaked hankerchief dating to this time. The gourd was allegedly a gift to Napoleon Bonaparte who became First Consul of France in 1799 and Emperor in 1804. An anonymous Italian family was in its posession since possibly the late 1800s and came forward with the relic. It bears an inscription that Maximilien Bourdaloue on 21. January dipped his hankerchief in the blood of the king. Dried blood was scraped out and this is the same DNA we present in this DNA spotlight! The sample contains unsually high and rare markers for the Y-DNA haplogroup G2a.

Louis XVI's direct male line ancestor Henri IV was famous for enacting the Edict of Nantes which guaranteed religious liberties to Protestants ending 30 years of fighting between French Protestants and Catholics - he was assassinated in 1610 by a French Catholic zealot. The remains had been presumed lost in the chaos of the French Revolution after a mob of revolutionaries desecrated the graves of French kings in the royal chapel of Saint-Denis in Paris in 1793. However, the head was passed down over the centuries by secretive private collectors and positively identifed in 2010 with a radiocarbon date between 1450 and 1650. The features were consistent with the king's face including a dark mushroom-like lesion near the right nostrial, a healed facial stab wound and a pierced right earlobe. The hair color and moustache and beard on the mummified head fit the appearance of the king at the time of his death as well as matched his portraits. Furthermore cutting wounds were visible corresponding to the separation of the head from the body in 1793 and digital facial reconstruction of the skull matched the plaster mould of his face made just after his death in 1610. The DNA was then tested and compared to the blood from the gourd.

The Roman conquest of Britain began in 43 AD but resistance in the north was fierce. Roman General Quintus Petillius Cerialis led the 9th Legion into the north and founded Eboracum in 71 AD (which became York) Originally Eboracum was intended to be a military fortress aligned along the river Ouse measuring about 50 acres in size. This wooden camp was upgraded to stone in 108 AD and garrisoned by the 6th Legion. The famous Emperor Hadrian reportedly visited Eboracum in 122 AD in order to plan his great walled frontier, which would be named after him. Emperor Septimus Severus visited in 208 AD and made it his private base while campaigning against Scotland, and he became the first of three Roman Emperors who would die in Eboracum. In 237, the town became a colonia, the highest legal status any Roman city could attain as Eboracum was the largest town in the north and the capital of Britannia Inferior. This is exactly the time period from when these 7 gladiators hailed.

Detailed analysis of these gladiators from York revealed some fascinating results. The bones showed various degrees of wear and tear as one might expect from the dangerous sport: 6DRIF-18 revealed a spinal fracture of the first vertibrae, 6DRIF-21, 6DRIF-3, and 3DRIF-16 meanwhile have fractured forearms, ankles and wrists. 6DRIF-22 has a skull injury as well as a stab to the neck - his extra vertebrae did not seem to assist with his fate. 6DRIF-23 meanwhile had 4 cuts to his jaw and was fully decapitated - clearly not the best fate to have. Last but not least 3DRIF-26 is fascinating indeed - he had a left shoulder injury, fractured ribs, damage wrists - and from a genetic standpoint is a deviation from the rest. His background compared to ancient samples from the time period matches very close to Ptolemaic Egyptians or the Near East.

The Crusader States (also known as Outremer) were created after the First Crusade (1095-1102) as a way to keep hold of territorial gains by Christian armies in the Middle East. Crusader castles were built all over Outremer to serve not only as defensive structures, but also as administrative and economic centers designed to last for many years to come. The four small Outremer states were the Kingdom of Jerusalem, the County of Edessa, the Country of Tripoli and the Principality of Antioch. This analysis focuses on the former Phoenician and later Roman colony of Sidon / Saida which remained in Arab hands until 1110 when King Baldwin I of Jerusalem and King Sigured I of Norway captured it. The city was then re-captured by the masterful Saladin in 1187, only to be re-taken by German Crusaders in 1197. It remained a key Crusader stronghold until the Saracen invasion of 1249 when it was destroyed - this series of exchanges presented the backdrop for the ill-fated 7th Crusade.

The 7th Crusade was led by Louis IX of France from 1248 to 1254. Jerusalem had recently fallen and there was no popular enthusiasm at the time for a new crsusade, as Europe was involved in many internal conflicts. Bela IV of Hungary was rebuilding his kingdom from ashes after the devastating Mongol invasion of Europe. Henry III of England was struggling at home and Haakon IV of Norway was in the midst of a civil war. Louis IX was almost alone in declaring a new crusade to the east and in 1248 sailed from Aigues-Mortes and Marseilles with an army of 15,000. They sailed for Cyprus but Louis decided to focus first on attacking Egypt. While marching towards Cairo, Louis' main force was attacked by Mameluk Baibars and defeated. In 1250 while attempting to return to a safer location, Louis himself was captured and taken prisoner after his army was annihilated. He was ransomed for 800,000 bezants after which he fled to Acre, one of the safest crusader strongholds in Outremer. The French King re-enforced local defenses and moved north to Sidon.