In 1907, two mummies were discovered in Deir Rifeh Egypt in a tomb belonging to a governor and his sons from the 12th Dynasty (almost 4000 years ago). The tomb group is one of the best preserved and best known burials of the Egyptian Middle Kingdom. Although the mummies were heavily decayed, the skeletons were still preserved. Khnum-Nakht was about 40 years old and Nakht-Ankh about 60.

The two mummies were found to be brothers with the same mother but different fathers. This is confirmed by identical mtDNA and the detected differences in the Y chromosomes. Both suffered from osteoarthtritis and dental attrition. Khnum-Nakht had kyphoscoliosis and Nakht-Ankh had lung lesions from sand pneumoconiosis.

The Birka female Viking warrior was a woman buried in the 10th century in Birka, Sweden and discovered in the 1870s. The grave was assumed to be a battle hardened man for 128 years until DNA analysis proved she was actually a high-ranking professional warrior. Shield-maidens are female warriors that take on male roles including wielding weapons and are depicted in many period figures, tapestries and brooches as well as mentioned by the famous Danish historian of the time, Saxo Grammaticus.

The grave chamber was made of wood and approximately 3.45m long and 1.75m wide. The body was in a sitting position and found earing garments of silk and silver. The grave contained a sword, an axe, a spear, armor-piercing arrows, a battle knife, two shields, two horses, one mare and one stallion. The grave also contained a game set with a board and pieces. This is considered evidence of her strategic thinking and indicating she was an officer who could lead troops into battle.

French revolutionists condemned King Louis XVI to death on 21. January 1793 by means of the guillotine at the Place de la Revolution in Paris (roughly where the Obelisk decorating the Place de la Concorde stands today). After a short but defiant speech he lost his head as the crowd rushed to the scaffold to dip hankerchiefs into his blood as momentos. An ornate gourd decorated with French Revolution themes was recently uncovered which had contained a blood soaked hankerchief dating to this time. The gourd was allegedly a gift to Napoleon Bonaparte who became First Consul of France in 1799 and Emperor in 1804. An anonymous Italian family was in its posession since possibly the late 1800s and came forward with the relic. It bears an inscription that Maximilien Bourdaloue on 21. January dipped his hankerchief in the blood of the king. Dried blood was scraped out and this is the same DNA we present in this DNA spotlight! The sample contains unsually high and rare markers for the Y-DNA haplogroup G2a.

Louis XVI's direct male line ancestor Henri IV was famous for enacting the Edict of Nantes which guaranteed religious liberties to Protestants ending 30 years of fighting between French Protestants and Catholics - he was assassinated in 1610 by a French Catholic zealot. The remains had been presumed lost in the chaos of the French Revolution after a mob of revolutionaries desecrated the graves of French kings in the royal chapel of Saint-Denis in Paris in 1793. However, the head was passed down over the centuries by secretive private collectors and positively identifed in 2010 with a radiocarbon date between 1450 and 1650. The features were consistent with the king's face including a dark mushroom-like lesion near the right nostrial, a healed facial stab wound and a pierced right earlobe. The hair color and moustache and beard on the mummified head fit the appearance of the king at the time of his death as well as matched his portraits. Furthermore cutting wounds were visible corresponding to the separation of the head from the body in 1793 and digital facial reconstruction of the skull matched the plaster mould of his face made just after his death in 1610. The DNA was then tested and compared to the blood from the gourd.